In Situ Loading of Basic Fibroblast Growth Factor Within Porous Silica Nanoparticles for a Prolonged Release

In Situ Loading of Basic Fibroblast Growth Factor Within Porous Silica Nanoparticles for a Prolonged Release

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Abstract Dishwasher Lower Tray Basic fibroblast growth factor (bFGF), a protein, plays a key role in wound healing and blood vessel regeneration.However, bFGF is easily degraded in biologic systems.Mesoporous silica nanoparticles (MSNs) with well-tailored porous structure have been used for hosting guest molecules for drug delivery.Here, we report an in situ route to load bFGF in MSNs for a prolonged release.The average diameter (d) of bFGF-loaded MSNs is 57 ± 8 nm produced by a water-in-oil microemulsion method.

The in vitro releasing profile of bFGF from MSNs in phosphate buffer saline has been monitored for 20 days through a colorimetric enzyme linked immunosorbent assay.The loading efficiency of bFGF in MSNs is estimated at 72.5 ± 3%.In addition, the Fridge Door Reed Switch cytotoxicity test indicates that the MSNs are not toxic, even at a concentration of 50 μg/mL.It is expected that the in situ loading method makes the MSNs a new delivery system to deliver protein drugs, e.

g.growth factors, to help blood vessel regeneration and potentiate greater angiogenesis.